Thomas Tuchel has insisted clubs "have to trust" England to handle players with sufficient care after Alex Scott and Ezri Konsa became the latest injury victims of the current international break.
Bournemouth midfielder Scott suffered a thigh problem against Croatia which is expected to rule him out for several weeks while Arsenal defender Konsa picked up a "hit on his quad" in the same game.
Manchester City's Nico O'Reilly left the camp last week while Tino Livramento, Kobbie Mainoo, Cole Palmer, Marcus Rashford and Declan Rice all withdrew from the initial squad due to either injury concerns or load management.
Bournemouth are reportedly unhappy with England's use of Scott amid suggestions he felt an issue in his thigh prior to playing against Croatia while Tuchel admitted that Konsa felt discomfort prior to joining up with the squad last month.
Asked about the difficulty of managing relations with clubs, Tuchel said: "I'm not involved in discussions with clubs how to use them. I think the players are for two-and-a-half weeks our players and as much as we trust the clubs to put the players' health first, I think the clubs have to trust us that we put the players' health first.
"You can see it in our team selections and that will never change but we are not on the phone to clubs asking for advice because then it would be an empty camp by now. It is four matches for us and this is the most important thing.
"For these two-and-a-half weeks, these four matches matter more than anything else. It is an honour, a pride to play for your country. This is where the focus is. The players are top professionals.
"For the players it means very, very much. You can see it in the way we play, in the way the group behaves and this is the spirit we want to have. Players are happy to stay and make themselves available, players are desperate to come into camp. This is exactly what we want. This is the only way forward."
Regarding Bournemouth's unhappiness over Scott, Tuchel said: "With Alex, it's a bit of strange one. I'm very sad about it.
"Alex trained both training sessions before the match full against Croatia. He did extra work after the training sessions.
"We knew that he was in treatment, but there were only green lights from himself, from the medics.
"When we asked him, when we were aware that there is a slight discomfort throughout the match, we asked him if he wants to come off. He said no.
"After the match, he said like, 'yeah, OK, it was the right thing to do, but I could have I could have played 90 minutes, I don't feel an injury' and even on the day after here before we did the scan, he felt that he's not injured, so it's a strange one because it does not match the objectivity of the of the scan and the prediction of the time that he is obviously going to miss.
"It doesn't match his feelings and doesn't match at all the process into the match and during the match.
"There was zero risk taking from our side. There was no awareness that there is any risk of something could happen like this. It's a kind of a one-off, strange one.
"I can 100% understand that Bournemouth is not happy and sad because they lose a key player."
Tuchel explained that Konsa has been battling for full fitness throughout.
"Ezri felt discomfort when he came into camp, played with discomfort against Spain, and it got a bit worse," he said.
"So, he stayed in camp and tried everything for the match in Czechia. That came too soon. Then he tried everything for the match in in Croatia and gave green light. Finished all the training sessions in between and was ready to play.
"Unfortunately, he got another hit on the quad and got a light trauma, and we decided to not let him play through this pain and discomfort again, so he left camp to get treatment for both his issues now."
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Harry Kane is set to equal Peter Shilton's all-time England appearance record of 125 caps in Tuesday's Nations League clash with Czechia at Wembley.
He took his tally to a record-extending 89 goals with the opener in Saturday's 7-0 thrashing of Croatia.
"Everything he does, he does it on the highest level, he does it consecutively and I am very sure he will be the one leading the team out onto the training pitch, he will lead the team in the warm-up and not do one step less or one step slower," Tuchel said.
"That is the level he has. He won't be one step slower. That's just his level.
"Since he took this new role for Bayern Munich he has found a new level. All these No 9s...They are a little different. They do it for the team but also for the records. That's just what drives them. You won't find any goalscorer of this calibre who isn't pushing it to the limit.
"That's how Harry's built. He scores all the time."