Cole Palmer, Declan Rice and Marcus Rashford have withdrawn from England duty ahead of their four Nations League matches.
Tino Livramento and Kobbie Mainoo have also been ruled out of England's upcoming games.
James Garner and Morgan Gibbs-White have been called up in their place and will report to St. George's Park on Tuesday
Palmer missed out on the World Cup squad and was set to play a key role in England's matches with Spain, Croatia and their home and away ties with Czechia. He's started the season in brilliant form for Chelsea but he will have to wait to make his England return as he has withdrawn with a minor muscular injury.
Rashford and Mainoo both featured in Manchester United's 1-1 draw with Fulham on Sunday.
Rashford was substituted in the 77th minute, while Mainoo was withdrawn in the 80th minute. Livramento was selected for the World Cup but has been forced to miss out on the eve of the tournament.
He's made two substitute appearances this season for Newcastle but picked up an injury in training on Friday.
"He [Livramento] suffered some discomfort in the morning after yesterday's training," Newcastle United manager Matthias Jaissle said post-match on Saturday.
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"At the moment, we should already have the results from the MRI, but there is nothing more from my end."
Thomas Tuchel is already without several players for the forthcoming batch of matches with Ben White, Dan Burn, Dean Henderson, Reece James, John Stones and Djed Spence all injured.